This week I have run my experiment 4000, 10000, and 1000000000 times. The results are shown below (aside from the 1000000000 iterations which is still running).
4000 Iterations
4000 Iterations
10000 Iterations
I have compared my results with mRNA Optimiser which continues to provide inconsistent results in comparison to Mfold.
mRNA Optimiser ∆G for a sequence:
mRNA Optimiser ∆G for a sequence:
Mfold ∆G for the same sequence: