For nearly this entire week I have been struggling to understand how to properly use D-Tailor's minimum free energy prediction software. After finally getting it work it, it predicted that the MFE is -2.2, which is very frustrating because it is significantly different than the results from mRNA Optimiser (-8256.7) and Mfold (-41).
I've also began writing up an abstract documenting my results to submit to the ISBRA Conference. However, I need to find other results to compare my results to complete it.
UPDATE: I had access to a PC this evening and was able to install and run Visual Gene Developer. The optimized sequence had a dG = -63.6. These results make the sense in relation to my program's result and provide the most sound comparison.
UPDATE: I had access to a PC this evening and was able to install and run Visual Gene Developer. The optimized sequence had a dG = -63.6. These results make the sense in relation to my program's result and provide the most sound comparison.